Multiplex Fluorescent in situ Hybridization
Multiplex Fluorescent in situ Hybridization
In collaboration with the McGinnis group (UCSD, CDB) and Terry Hwa's lab (UCSD, Physics) we have also developed new methods and quantitative tools to detect multiple gene expression patterns in single cells using multiplex in situ hybridization [23] as well as computational techniques to quantitatively measure gene expression levels [24]. We have applied these to generate testable models of pattern formation in both the developing embryo and wing. For example, most recently, we have proposed that differences in gene length alone can create a transient nested pattern of gene expression to help “seed” morphogen-dependent patterning across a developing field of cells [24]. We have also developed bioinformatic tools for mining and comparing genome data [25, 26].
http://people.biology.ucsd.edu/davek/
In collaboration with the McGinnis group (UCSD, CDB) and Terry Hwa's lab (UCSD, Physics) we have also developed new methods and quantitative tools to detect multiple gene expression patterns in single cells using multiplex in situ hybridization [23] as well as computational techniques to quantitatively measure gene expression levels [24]. We have applied these to generate testable models of pattern formation in both the developing embryo and wing. For example, most recently, we have proposed that differences in gene length alone can create a transient nested pattern of gene expression to help “seed” morphogen-dependent patterning across a developing field of cells [24]. We have also developed bioinformatic tools for mining and comparing genome data [25, 26].
http://people.biology.ucsd.edu/davek/
Triple Fluorescent mRNA
Multiplex Fluorescent mRNA In Situ Hybridization
9/26/03: This site is under construction, but is nearly complete. It presents information on how to do multiplex fluorescent mRNA in situ hybridization on Drosophila embryos. Questions, suggestions, or comments, please e-mail me at davek@biomail.ucsd.edu. The following links provide protocols, images, and general information on this project: Text needed here?
9/26/03: This site is under construction, but is nearly complete. It presents information on how to do multiplex fluorescent mRNA in situ hybridization on Drosophila embryos. Questions, suggestions, or comments, please e-mail me at davek@biomail.ucsd.edu. The following links provide protocols, images, and general information on this project: Text needed here?
